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IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI
《农业科学与工程前沿（英文）》 2021年 第8卷 第4期 页码 635-644 doi: 10.15302/J-FASE -2020343
The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of Eucalyptus tereticornis, Xanthium sibiricum, Artemisia argyi, Tupistra chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plant pathogenic fungi Aspergillus niger, Botrytis cinerea, Penicillium digitatum, P. expansum, P. italicumand Rhizopus stolonifer. All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v), 95% ethanol (v/v), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. T. chinensis may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.
关键词： Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis
An introduction to the medicinal plant genome project
Shilin Chen, Li Xiang, Xu Guo, Qiushi Li
《医学前沿（英文）》 2011年 第5卷 第2期 页码 178-184 doi: 10.1007/s11684-011-0131-0
ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI
Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG
《农业科学与工程前沿（英文）》 2021年 第8卷 第4期 页码 635-644 doi: 10.15302/J-FASE-2020343
关键词： Botrytis cinerea food preservation plant diseases plant pathogenic fungi Tupistra chinensis
The regulatory sciences for stem cell-based medicinal products
null
《医学前沿（英文）》 2014年 第8卷 第2期 页码 190-200 doi: 10.1007/s11684-014-0323-5
Over the past few years, several new achievements have been made from stem cell studies, many of which have moved up from preclinical stages to early, or from early to middle or late, stages thanks to relatively safe profile and preliminary evidence of effectiveness. Moreover, some stem cell-based products have been approved for marketing by different national regulatory authorities. However, many critical issues associated mainly with incomplete understanding of stem cell biology and the relevant risk factors, and lack of effective regulations still exist and need to be urgently addressed, especially in countries where establishment of appropriate regulatory system just commenced. More relevantly, the stem cell regulatory sciences need to be established or improved to more effectively evaluate quality, safety and efficacy of stem cell products, and for building up the appropriate regulatory framework. In this review, we summarize some new achievements in stem cell studies, especially the preclinical and clinical studies, the existing regulations, and the associated challenges, and we then propose some considerations for improving stem cell regulatory sciences with a goal of promoting the steadfast growth of the well-regulated stem cell therapies abreast of evolvement of stem cell sciences and technologies.
关键词： stem cell-based medicinal products (SCMPs) stem cell therapy (SCT) safety effectiveness standards guidelines regulatory science
Optimizing hairy root production from explants of
Zhaogui YAN, Shengyu LIU, Junlian ZHANG, Guan HUANG, Lijun DUAN, Yaomei YE
《农业科学与工程前沿（英文）》 2020年 第7卷 第4期 页码 513-522 doi: 10.15302/J-FASE-2019272
is a shrub that has been used in traditional herbal medicine. It has great pharmaceutical potential for treating diseases such as cancer and diabetes. As a prerequisite for propagation of this species on a large scale, hairy roots in were induced using and various factors affecting hairy root induction and growth evaluated. Seven factors were tested: (1) type of explant, (2) type of culture medium, (3) duration of pre-culture, (4) inoculum cell density, (5) duration of infection, (6) acetosyringone concentration in the culture medium, and (7) duration of incubation. The optimal protocol for hairy root induction and growth was: young shoots, pre-cultured in Y1 for 2 d, inoculated with broth with an OD of 0.6 for 20 min, and incubated for 3 d. Putative transgenic hairy roots were initially identified by morphology and then confirmed by polymerase chain reaction. Successful and optimal production of hairy roots is a critical prerequisite for industrial scale clonal propagation of . Being able to cultivate the plant on a large scale will provide rapid and ready supply of the plant materials that can be used in herbal medicine and in scientific and industrial exploitation.
关键词： hairy root induction medicinal plant MS growth medium Phyllanthus hainanensis Rhizobium rhizogenes
T. G. YUDINA, Danyang GUO, N. F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA
《化学科学与工程前沿（英文）》 2012年 第6卷 第2期 页码 203-209 doi: 10.1007/s11705-012-1277-2
关键词： recombinant destabilase-lysozyme (rec.Dest-Lys) antimicrobial activity antifungal activity medicinal leech heated-up derivative of rec.Dest-Lys (T-rec.Dest-Lys)
HEMIPTERAN-TRANSMITTED PLANT VIRUSES: RESEARCH PROGRESS AND CONTROL STRATEGIES
《农业科学与工程前沿（英文）》 2022年 第9卷 第1期 页码 98-109 doi: 10.15302/J-FASE-2021389
About 80% of plant viruses are transmitted by specific insect vectors, especially hemipterans with piercing-sucking mouthparts. Many virus-transmitting insects are also important crop pests that cause considerable losses in crop production. This review summarizes the latest research findings on the interactions between plant viruses and insect vectors and analyzes the key factors affecting insect transmission of plant viruses from the perspectives of insect immunity, insect feeding, and insect symbiotic microorganisms. Additionally, by referring to the latest applications for blocking the transmission of animal viruses, potential control strategies to prevent the transmission of insect-vectored plant viruses using RNAi technology, gene editing technology, and CRISPR/Cas9+ gene-driven technology are discussed.
关键词： control strategies / feeding / immunity / insect vector / microorganism / plant virus
Intelligent diagnosis methods for plant machinery
Huaqing WANG, Peng CHEN, Shuming WANG,
《机械工程前沿（英文）》 2010年 第5卷 第1期 页码 118-124 doi: 10.1007/s11465-009-0084-z
关键词： intelligent diagnosis neural network fuzzy algorithm adaptive filtering plant machinery
Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction
Hong LI,Ziding ZHANG
《农业科学与工程前沿（英文）》 2016年 第3卷 第2期 页码 102-112 doi: 10.15302/J-FASE-2016100
关键词： plant–pathogen interactions systems biology omics plant immunity protein–protein interaction network
Causations of failure in megaprojects: A case study of the Ajaokuta Steel Plant project
Oluwole Alfred OLATUNJI
《工程管理前沿（英文）》 2018年 第5卷 第3期 页码 334-346 doi: 10.15302/J-FEM-2018011
This paper uses project organizational theories to draw lessons from a historic megaproject, the Ajaokuta Steel Plant (ASP). Archival reports on the ASP were explored to identify the unique attributes of the project; the political wrangling that underplayed its evolution, its economic significance and organizational impacts. Findings suggest the goals of the ASP project were, and still are, unambiguous. Failure occurred as socio-political forces aggravated the project’s complex milestones. Stakeholders were impatient with pre-project investigations. During planning, owners ignored opinions that were contrary to their expectations. While delays lingered, pressures from the global economy weakened the project’s motivation to succeed. These combined to turn the project’s outcomes into a chaotic situation that triggered dire implications. Despite about 1400% overrun in cost, the success achieved on the plant was 28% at commissioning. Contractors remained on site until eight years after commissioning. Six key elements of the 482 items in the ASP project contract were not delivered nearly 40 years on. A simplistic look at these suggests poor planning is the main problem. However, planning issues is not entirely strange in greenfield projects. The paper draws strength from project organization theories to explain what was poor about the planning. Socrates’ generic management theory was used to explain the role of leadership in the failure of the ASP project. McGregor’s Theory X and Theory Y explain the significance of stakeholders’ integration in megaprojects. Systems and chaos theories were used to explain the sensitivity of the ASP project to uncertainties. Narratives on these combine well to inspire stakeholders of megaprojects on where and how to seek courage in making effective plans that can help achieve success in complex projects. While normative literature only recognizes project success in a definitive perspective, this study provides insights from failure as an instrument to trigger sublime reflections.
关键词： industrial projects megaprojects Nigeria project organizational theories steel plant
Rhizosphere immunity: targeting the underground for sustainable plant health management
Zhong WEI, Ville-Petri FRIMAN, Thomas POMMIER, Stefan GEISEN, Alexandre JOUSSET, Qirong SHEN
《农业科学与工程前沿（英文）》 2020年 第7卷 第3期 页码 317-328 doi: 10.15302/J-FASE-2020346
Managing plant health is a great challenge for modern food production and is further complicated by the lack of common ground between the many disciplines involved in disease control. Here we present the concept of rhizosphere immunity, in which plant health is considered as an ecosystem level property emerging from networks of interactions between plants, microbiota and the surrounding soil matrix. These interactions can potentially extend the innate plant immune system to a point where the rhizosphere immunity can fulfil all four core functions of a full immune system: pathogen prevention, recognition, response and homeostasis. We suggest that considering plant health from a meta-organism perspective will help in developing multidisciplinary pathogen management strategies that focus on steering the whole plant-microbe-soil networks instead of individual components. This might be achieved by bringing together the latest discoveries in phytopathology, microbiome research, soil science and agronomy to pave the way toward more sustainable and productive agriculture.
关键词： rhizosphere soil microbiome plant immunity microbial ecology plant health soilborne pathogens
ROUTE DEVELOPMENT, ANTIVIRAL STUDIES, FIELD EVALUATION AND TOXICITY OF AN ANTIVIRAL PLANT PROTECTANT
《农业科学与工程前沿（英文）》 2022年 第9卷 第1期 doi: 10.15302/J-FASE- 2021390
It has previously been shown that tryptophan, the biosynthesis precursor of Peganum harmala alkaloids, and its derivatives have anti-TMV activity both in vitro and in vivo. Further exploration of this led to the identification of NK0238 as a highly effective agent for the prevention and control of diseases caused by plant viruses, but the existing routes are unsuitable for its large-scale synthesis. This study optimized a route for two-step synthesis of this virucide candidate via reaction of l-tryptophan with triphosgene to produce l-tryptophan-N-carboxylic anhydride, which then reacts with n-octylamine to give NK0238 at up to 94% yield and nearly 97% HPLC purity. In addition, the route was used for the preparation of NK0238 on a>40 g scale permitting further assessment of its antivirus activity in the greenhouse and field experiments, and toxicity tests. NK0238 exhibited useful antiviral activities against a variety of viruses both in greenhouse and field experiments. The toxicity tests showed that NK0238 was not acutely toxic to birds, fish, honey bees and silkworms. The optimized route provides a solid foundation for its large-scale synthesis and subsequent efficacy and toxicity studies, its excellent activity and safety make NK0238 a promising drug candidate for further development.
Yonghua CHEN, Xiaofu WU, Mingli CHEN, Kelin LI, Jing PENG, Peng ZHAN
《环境科学与工程前沿（英文）》 2013年 第7卷 第6期 页码 906-912 doi: 10.1007/s11783-013-0579-z
关键词： vertical-flow constructed wetlands plant intercropping model warm seasonal plant model
《农业科学与工程前沿（英文）》 2022年 第9卷 第3期 页码 313-315 doi: 10.15302/J-FASE-2022460
Regulation of NLR stability in plant immunity
Tao WANG, Jiaxin LI, Qian-Hua SHEN
《农业科学与工程前沿（英文）》 2019年 第6卷 第2期 页码 97-104 doi: 10.15302/J-FASE-2018248
Plant nucleotide binding domain and leucine-rich repeat (NLR) receptors recognize pathogen effectors directly or indirectly and mediate innate immune responses. NLR-mediated immunity also has direct impacts on plant growth and development, as well as yield and survival. The levels of NLR proteins are therefore intricately controlled in plants to balance defense responses and other processes. In recent years, the ubiquitination-26S proteasome system and the HSP90 chaperones have emerged as having key functions in the regulation of NLR stability. The N-end rule pathway of protein degradation is also directly linked to NLR stability. Recent progress in the regulation of NLR stability and turnover is summarized here, focusing on the key components and pathways.
关键词： E3 ubiquitin ligase degradation nucleotide-binding leucine-rich repeat receptor plant immunity proteasome protein stability ubiquitination
标题 作者 时间 类型 操作
ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI
Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG
期刊论文
Optimizing hairy root production from explants of
Zhaogui YAN, Shengyu LIU, Junlian ZHANG, Guan HUANG, Lijun DUAN, Yaomei YE
期刊论文
Antifungal and antibacterial functions of medicinal leech recombinant destabilase-lysozyme and its heated-up
T. G. YUDINA, Danyang GUO, N. F. PISKUNKOVA, I. B. PAVLOVA, L. L. ZAVALOVA, I. P. BASKOVA
期刊论文
Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction
Hong LI,Ziding ZHANG
期刊论文
Causations of failure in megaprojects: A case study of the Ajaokuta Steel Plant project
Oluwole Alfred OLATUNJI
期刊论文
Rhizosphere immunity: targeting the underground for sustainable plant health management
Zhong WEI, Ville-Petri FRIMAN, Thomas POMMIER, Stefan GEISEN, Alexandre JOUSSET, Qirong SHEN
期刊论文
ROUTE DEVELOPMENT, ANTIVIRAL STUDIES, FIELD EVALUATION AND TOXICITY OF AN ANTIVIRAL PLANT PROTECTANT
期刊论文
Decontamination efficiency and root structure change in the plant-intercropping model in vertical-flow
Yonghua CHEN, Xiaofu WU, Mingli CHEN, Kelin LI, Jing PENG, Peng ZHAN
期刊论文
HIGHLIGHTS OF THE SPECIAL ISSUE “PROGRESS ON NITROGEN RESEARCH FROM SOIL TO PLANT AND TO THE ENVIRONMENT
期刊论文
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